Jurkat T cells and development of the T-cell receptor ... - Nature
Apr 1, 2004 · Perhaps the best known of these model systems is the Jurkat leukaemic T-cell line, and here we review some of the key advances in the field of TCR signalling that were made with Jurkat T cells...
T cell activation and effector function in the human Jurkat T cell ...
The CD4 T cell line Jurkat (JK) has been widely employed to investigate intracellular signaling leading to T cell activation in response to T cell receptor (TCR) triggering.
Jurkat cells - Wikipedia
Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. [1]
A T-cell reporter platform for high-throughput and reliable ...
Nov 23, 2020 · Here, we propose a Jurkat-based triple parameter TCR signalling reporter endogenous TCR knockout cellular platform (TPR KO) that offers an unbiased, easy read-out of TCR functionality and facilitates high-throughput screening approaches.
Using the Jurkat reporter T cell line for evaluating the functionality ...
Feb 21, 2023 · The human Jurkat T cell line has been an important research tool for interrogating the function of the T cell receptor (TCR)s (Aarnoudse et al., 2002; Anmole et al., 2015; Rosskopf et al., 2018; Müller et al., 2020).
CRISPR/Cas9-medaited knockout of endogenous T-cell receptor in Jurkat …
Sep 1, 2022 · In our study, the TCR-KO Jurkat cells were engineered by lentiviral transduction to express HLA-A2*0201-restricted TCRs specific for the SLLMWITQC NY-ESO-1 peptide. We assessed the functionality of the transgenic TCRs in terms of …
A Jurkat 76 based triple parameter reporter system to evaluate TCR ...
Apr 3, 2018 · Here, we describe the generation of triple parameter reporter T cells based on the Jurkat 76 T cell line for the evaluation of TCR and chimeric antigen receptor functions as well as adoptive T cell strategies.
Synthetic TRuC receptors engaging the complete T cell receptor …
May 7, 2019 · Jurkat T cells expressing the HA1.7 TCR (Jurkat cell line CH7C17) were transduced with a control vector (black) or vectors encoding for the ε-TRuC (light blue), the 28ζ (red) or BBζ CAR...
High-throughput pairing of T cell receptor α and β sequences
As a positive control for pairing, a human T cell leukemia (T-ALL) cell line known to be positive for αβ TCR, Jurkat clone E6-1 (ATCC TIB-152) , was spiked into the cell suspension of experiment 1 at a ratio of 1 Jurkat cell per ~30,000 total cells.
High-throughput targeted long-read single cell sequencing
Jul 16, 2019 · We show that Repertoire and Gene Expression by Sequencing (RAGE-Seq) can generate accurate full-length antigen receptor sequences at nucleotide resolution, infer B-cell clonal evolution and...