Expansion microscopy of C. elegans - PMC - National Center for ...
May 1, 2020 · Here we present a strategy, expansion of C. elegans (ExCel), to expand fixed, intact C. elegans. ExCel enables simultaneous readout of fluorescent proteins, RNA, DNA location, and anatomical structures at resolutions of ~65–75 nm (3.3–3.8x linear expansion).
Expansion microscopy of C. elegans | eLife
May 1, 2020 · Here we present a strategy, expansion of C. elegans (ExCel), to expand fixed, intact C. elegans. ExCel enables simultaneous readout of fluorescent proteins, RNA, DNA location, and anatomical structures at resolutions of ~65–75 nm (3.3–3.8x linear expansion).
The Caenorhabditis elegans Transgenic Toolbox - PMC
Aug 12, 2019 · Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression.
CaeNDR, the Caenorhabditis Natural Diversity Resource
Oct 19, 2023 · Here, we present an updated platform, the Caenorhabditis Natural Diversity Resource (CaeNDR), that enables quantitative genetics and genomics studies across the three Caenorhabditis species: C. elegans, C. briggsae and C. tropicalis.
Transparent Window into Biology: A Primer on Caenorhabditis elegans …
Jun 3, 2015 · C. elegans has a rapid life cycle (3 days at 25° from egg to egg-laying adult) and exists primarily as a self-fertilizing hermaphrodite, although males arise at a frequency of <0.2% . These features have helped to make C. elegans a powerful model of …
Cryosectioning and immunofluorescence of C. elegans reveals …
2 days ago · Polyphosphate (polyP), a ubiquitous and highly conserved molecule, is present throughout all branches of life yet has mostly been studied in single cells. Quarles et al. describe a method to longitudinally section Caenorhabditis elegans, allowing high-throughput staining of worms using polyP-specific fluorescent probes. These studies establish C. elegans as a model for polyP research.
See Elegans: Simple-to-use, accurate, and automatic 3D detection …
Mar 22, 2024 · We introduce See Elegans, an algorithm for automatic detection, tracking, and identification of C. elegans neurons, incorporating both sequence-dependent (as in [12, 14, 28]) and sequence-independent methods (as in [13, 29]) to …
Caenorhabditis elegans model studies show MPP+ is a simple …
May 5, 2021 · Studies with Cat-2 (the C.elegans ortholog of mammalian tyrosine hydroxylase) and human α-synuclein over-expressing transgenic C. elegans strains suggest that intracellular catecholamine and α-synuclein may contribute to the overproduction of ROS.
CemOrange2 fusions facilitate multifluorophore subcellular imaging in C ...
Mar 26, 2019 · We have expanded the repertoire of fluorescent proteins for use in C. elegans by developing a codon-optimized version of Orange2 (CemOrange2). Proteins or targeting motifs fused to CemOrange2 were distinguishable from the more common fluorophores used in the nematode; such as GFP, YFP, and mKate2.
C. elegans : A biosensor for host–microbe interactions - Nature
Mar 1, 2021 · The nematode Caenorhabditis elegans has been widely used as a model organism to study a remarkable number of human-like processes. Recent evidence shows that the worm is a powerful tool to...