A new method uses magnetic beads to hold molecules in place, keeping the sample intact and reducing sample loss by a ...

This process tethered the target molecules to the magnetic beads, and the magnetic beads to the grid. With the particles securely in place, blotting could then be done safely, with far less sample ...

This process tethered the target molecules to the magnetic beads, and the magnetic beads to the grid. With the particles securely in place, blotting could then be done safely, with far less sample ...

and the magnetic beads to the grid. With the particles securely in place, blotting could then be done safely, with far less sample loss. "In traditional cryo-EM, the target molecules just float ...

Thick multicellular plant samples provide unique challenges when it comes to cryo-preservation, which has resulted in limited successful examples for structural studies using in situ cryo-electron ...

The chromatin droplet solution was applied to the back side of the cryo-EM grid and rapidly vitrified using a highpressure freezer. The grid was then imaged with a cryo-fluorescence microscope to ...

The researchers then used a strong magnet to attract the magnetic beads to the cryo-EM grid membrane, which enabled them to generate sufficient cryo-EM data from a dilute sample. The MagIC-cryo-EM ...

Once the sample was applied, the excess solution was blotted for 3 s using a blot force of 20. Immediately after blotting, the grid was plunged into liquid ethane, rapidly freezing the sample and ...

For example, a biomolecular complex may prove to be unstable upon application to the cryo-EM grid, or upon exposure to the air-water interface. While we have no evidence that this is the case for the ...

Many proteins - key to the study of disease - are particularly difficult to crystallise. In cryo-EM, samples in an aqueous solution are applied to a grid mesh, then plunged into liquid ethane, which ...