One of the approaches is the use of insertion of genetic elements into the vector backbone which have shown ... variability in gene expression by controlling integration sites. Traditional homologous ...

Centriolar subdistal appendages and in particular their component CEP170 promote double‐strand break repair by homologous recombination (HR ... harboring a mCherry‐tagged PLK4, or an empty vector as a ...

Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1-driven homologous recombination by acting on different steps during filament assembly, reveal Tokyo Tech researchers in collaboration with National Taiwan ...

As new traits are added to existing transgenic lines, site-specific integration ... (control of recombination) recombinase not only deletes transgenic DNA no longer needed after transformation but ...

In contrast to all current genome editing platforms, AAV exclusively utilizes the high-fidelity homologous ... intracellular AAV vector genomes predominantly survive as episomes long-term (Figure 2A), ...

The Ad plasmid is generated by recombination of a shuttle ... provides long homology arms for the Ad∆E1 vector which can lead to double homologous crossover during the production process and ...

Here, we establish isogenic female/male ESCs and show that the female ESCs are more sensitive to camptothecin and have lower gene targeting efficiency than male ESCs, suggesting that homologous ...

Our vector series ... we show that short homology arm-directed targeted integration of a GFP reporter into safe harbor loci in porcine and human cells increased 10-fold in comparison to homologous ...

which is typically performed through homologous recombination, a complex, ineffective and costly process. In this study, we developed a multiplexable and universal nuclease-assisted vector integration ...

Using chromosomally integrated fluorescence reporter transgenes, we demonstrate a new recombinogenic activity of AID leading to intra- and intergenic deletions via homologous recombination ... had ...

A major bottleneck of current technology is the necessity to introduce transgenes by homologous recombination in ... 136 surviving clones showed correct integration at the 5′ and 3′ end.

Additionally, transgenes might be subjected to silencing due to chromosomal position effects; iii) random integration ... Correction by homologous recombination (HR) results in the reversion ...