2.6 Reaction condition optimization for multiplex real-time PCR The singleplex real-time PCR assays for CHV-1, CAdV-2, and CDV described above were multiplexed into one reaction consisting of Premix ...

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The resulting cDNA was subjected to real-time PCR analysis with Fast SYBR Green Master Mix (3485612, Applied Biosystems) in a Step One Plus Real-Time PCR System (Applied Biosystems). The abundance of ...

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