Somatic hypermutation (SHM) of immunoglobulin variable (V) regions modulates antibody-antigen affinity is initiated by activation-induced cytidine deaminase (AID) on single-stranded DNA (ssDNA).

The highly processive and novel warm-start reverse transcriptase allows scientists to run qPCR and PCR experiments without ice either right on the bench or out in the field. Once the lyophilized eQo ...

troubleshoot experiments, design primers and determine reaction conditions. We will cover critical requirements for amplification, thermostable DNA polymerases, reverse transcriptase reactions, ...

Quantitative reverse transcription PCR (RT-qPCR ... including Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) reporting, are in the Supporting Information. 2.3.

Reverse transcription real-time PCR has the disadvantage that it takes longer compared ... KSt conceptualized the study and designed the experiments. VM and KSi conducted the experiments. KSt, VM, and ...

For example, controls need to be included for contamination and, if applicable, errors in reverse transcription ... the design of experiments) called the Minimum Information for the Publication of ...

In the two-step method, the reverse transcription and the qPCR amplification occur sequentially as two separate experiments. In the one-step method, RT and qPCR are performed in the same tube. Digital ...

For this reason, our initial strand-displacement experiments with D732N employed ... step of 30 min at 68°C was employed to allow the reverse transcription, followed immediately by programmed PCR ...

This would result in positive PCR tests due to the continuing transcription ... RT experiment, which showed that cell lysates from cells expressing RT of either type could cause reverse ...

These two key events of the viral life cycle are commonly viewed as separate not only in time, but also in cellular space, since reverse transcription (RT ... Data are from one experiment. For CPSF6, ...