Like Pol theta, HIV reverse transcriptase acts as a DNA polymerase, but can also bind RNA and write RNA back into a DNA strand. In a series of elegant experiments, the researchers tested ...

Scientists have now created a synthetic reverse transcriptase using directed evolution ... copying RNA into DNA and then amplifying the DNA with a DNA polymerase. But ...

The enzyme RT of HIV-1 is responsible for copying the viral single-stranded RNA genome to double-stranded DNA (dsDNA ... acid duplex may slide past the polymerase active site 17, and a rearranged ...

RT-PCR (reverse-transcriptase polymerase chain reaction): PCR is a method that allows logarithmic amplification of short DNA sequences within a longer, double-stranded DNA molecule. Gene expression ...

Alzheimer's disease is the most common cause of dementia and affects more than a tenth of Americans aged 65 and older. The ...

Management and Outcome Differences in Supraglottic Cancer Between Ontario, Canada, and the Surveillance, Epidemiology, and End Results Areas of the United States Purpose: To determine whether reverse ...

The reverse transcriptase enzyme that catalyzes this reaction has RNase activity that then degrades the RNA portion of the hybrid. Subsequently, a single-stranded DNA molecule is synthesized by the ...

After removing the nucleos(t)ide analog reverse-transcriptase inhibitor monophosphate, the mode of DNA polymerization becomes active again (bottom). The P site and N site of HIV-1 reverse ...

“The idea of having a motor that you put in reverse sounds very appealing to us, but it's much more efficient to throw the motor out,” Wuite explained. Unlike cars, a cell has multiple DNA polymerase ...

Prior to its release into the nuclease, the covalently bound viral reverse transcriptase/DNA polymerase is removed. The nicked HBV DNA is repaired by the human host cell's DNA repair machinery.

In the latest shake-up for scientific dogma, research has demonstrated that eukaryotic cells can convert RNA sequences back into DNA, through a misunderstood, ‘error-prone’ polymerase. Historically, ...