The assembled R3T one-step qRT-PCR kit based on in-house, patent-free Taq Pol and MMLV-RT demonstrated similar reliability and sensitivity to those of the Invitrogen SuperScript III Platinum One-Step ...

Heat pretreatment could improve the RT-qPCR detectability of plasmid-borne ARGs in environmental samples by eliminating the supercoiled form of plasmids.

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