The spectrophotometer was used to measure the absorbance at 405 nm every minute for 30 min. The enzyme activity was calculated as nano-mol of para-nitrophenol per min (nmol pNP/min). The ALP activity ...

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In the presence of magnesium and zinc ions, p-nitrophenylphosphate is cleaved by phosphatases into phosphate and p-nitrophenol. The p-nitrophenol released is directly proportional to the catalytic ...

The objective of this study was to evaluate the effects of three levels of lipids in the diet on growth, metabolism, and intestinal and hepatic histopathology in discus fish (Symphysodon ...