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All the reagents and chemicals obtained from commercial sources were of analytical grade. Free amino acids were estimated using the ninhydrin method after 30, 60, 90, and 120 min-hydrolysis (Lie, 1973 ...
2020). For the determination of proline (Pro) by the acid ninhydrin method, the absorbance of the sample reacting with hydrochloric acid and ninhydrin to form a purple compound was measured at 570 nm ...
School of Chemistry and Biochemistry, Georgia Institute of Technology, 950 Atlantic Drive, Atlanta, Georgia 30332, United States Center for Microbial Dynamics and Infection, Georgia Institute of ...