Amidst the continued struggle to treat non-small-cell lung cancer, a new study led by Stanford University scientists suggests that a patient's response to immunotherapy may hinge on how immune cells ...

Together, these findings highlight the crucial role of TRPV1 signaling in monocyte migration and macrophage-related ... Finally, the samples were washed three times with PBS, mounted on slides, and ...

The Cleveland Browns finally selected Colorado Buffaloes quarterback Shedeur Sanders in the fifth round of the 2025 NFL Draft—four rounds later than he was initially predicted to go—but the ...

Monocyte differentiation in macrophages was hindered during ... For cytospins 200 µL of cell suspension/slide were centrifuged at 800 rpm for 5 min (Thermo Scientific™ Cytospin™ 4 cytocentrifuge, ...

Slides were dehydrated, mounted with Pertex (Histolab Products ... The present study investigated S100A12 dynamics in monocyte subsets, during monocyte maturation stages and polarization, as well as ...

This acute treatment was anti-inflammatory in vivo as shown by pre-treatment of monocytes prior to adoptive transfer into ... with apoA1 (40 µg/ml), or vehicle prior to plating in chamber slides and ...

The slides were stained with various primary antibodies ... To investigate whether the cultured cells were monocytes, we stained the cells with anti-CD68 antibodies (R&D Systems, dilution 1: ...

However, monocytes contribute to macrophages in the acute infarct ... Images were captured using a Nikon Eclipse 80i (Nikon Instruments), or slides were scanned with NanoZoomer 2.0-RS in ...

and seeded onto μ-Slide VI chambers (ibidi) coated with 0.2% (wt/wt) gelatin (Sigma-Aldrich). MAEC were stimulated for 20 h with either vehicle or 20 ng/mL human TNF-α (R&D Systems). Metabolic stress ...

This resulted in 8 sections per slide, with a distance of 80 μm apart ... data were normalized for individual mice based on the percentage of blood monocytes labeled. For example, if an individual ...

Monocytes were identified as CD11b(+) CD90(−) B220(−) DX5(−) NK1.1(−) Ly-6G (low). For morphologic characterizations, cells were spun and resuspended in PBS, and slides were prepared by ...