The Scientist1y
Insights into qPCR: Protocol, Detection Methods, and Analysis
View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to ...
eLife1y
Pleiotropic effects of BAFF on the senescence-associated secretome and growth arrest
Senescent cells release a variety of cytokines, proteases, and growth factors collectively known as the senescence-associated secretory phenotype (SASP). Sustained SASP contributes to a pattern of ...
The Scientist3y
An Optimized Culture Medium for Induced Pluripotent Stem Cells
and c-Myc. 1 Now, researchers can differentiate reprogrammed induced pluripotent stem cells (iPSCs) into any cell type and use them for numerous applications, including disease research, drug ...
Frontiers3y
The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro
The primers used in qPCR are listed in Table 1 ... In summary, we analyzed the duck TRIM25 gene and constructed the pcDNA3.1-c-myc-duTRIM25 plasmid to investigate the antiviral effect of TRIM25 on ...
eLife4y
MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor–1
The oncoprotein transcription factor MYC is a major driver of malignancy and a highly validated but challenging target for the development of anticancer therapies. Novel strategies to inhibit MYC may ...
Frontiers5y
Neddylation Facilitates the Antiviral Response in Zebrafish
The kidneys and spleens were collected and stored at −80°C for further qPCR assays. Myc-nedd8 and GFP were subcloned into Psp64 poly (A) vector (Promega). AmpliCap SP6 High Yield message maker kit ...