Mass General Brigham researchers are shining a powerful new light into the viral darkness with the development of ...

After the treatment time, the cells were lysed with the Nano-Glo Luciferase Assay Reagent (Promega) according to the manufacturer’s protocol, and luminescence was measured using the Tecan Spark ...

Luciferase assays were performed as described previously (67 ... proteomics screen and reverse transfected into MRC-5 cells according to the manufacturer protocol (DharmaFECT reverse transfection of ...

This is a protocol for a Cochrane Review (prognosis). The objectives are as follows: To determine the prognostic value of the Decipher gene assay in men with biopsy-confirmed clinically localized ...

Luciferase assays showing enhancer activity in the SNP rs4971059 region ... according to the manufacturer's protocol. Overall, 5% (1:20) cellular extracts were used for input. For immunoprecipitation, ...

and pseudovirus-based neutralization assay (Protocol 3) relying on reading quantitative luciferase luminescence signals. We utilized this system to conduct seroprevalence studies and measure nAbs in ...

Luciferase assay of Dct promoter cloned downstream of firefly luciferase ... according to manufacturer's protocol. 100–500 ng of RNA was taken for cDNA synthesis using Superscript III (Invitrogen, ...

Designed as a convenient, simple one-step, homogeneous protocol this Luciferase Assay System minimizes handling steps to support high-throughput screening applications. The One-Step Luciferase Assay ...