Tissue was mounted on Superfrost™ Plus slides (Thermo Fisher Scientific, New Zealand) in anti-fade solution (0.1 g p-phenylenediamine made with 80% (v/v) glycerol in 0.1 M phosphate buffer, pH 8.5).

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The optical density (OD) values of cells after cultivation for 1, 3, and 5 days were measured by Thermo Fisher Scientific Multiskan GO Spectrophotometer (Waltham, MA, United States) using Cell ...