The whole procedure, including protoplast isolation, takes approximately 6 h. ... 4% Ficoll solution Mix 4.5 ml vacuole buffer and 3 ml lysis buffer. Keep at room temperature ...

Here, we present a protocol for the isolation of endothelial cells (ECs) from tissues. ECs make up a minor population of cells in a tissue, but play a major role in tissue homeostasis, as well as ...

Biotechnological innovation meets horticulture as researchers unveil a cutting-edge genome editing protocol for grapes. This novel technique, integrating protoplast culture with CRISPR-Cas9 ...

Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by ...

Another significant finding when comparing the two technologies was that approximately 93% of Ficoll isolated monocytes were positive for platelet marker CD41/61. Of these, 88% were positive for ...

Isolation of parasites from feces or blood: ... (Ficoll 400) and a radiopaque contrast medium (Hypaque) in a specific ratio to yield a density of 1.114 ± 0.002 g/mL.