For our high-throughput screening campaign, we utilized an NSC34 motor neuron cell line expressing an SMN2 minigene reporter ... via qPCR for dose responsiveness and to confirm the desired splicing ...

The RNA‐binding protein Y14 binds preferentially to mRNAs produced by splicing and is a component of a multiprotein complex that assembles ∼20 nucleotides upstream of exon–exon junctions. This complex ...

We have used chemical footprinting, kinetic dissection of reactions and comparative sequence analysis to show that in self‐splicing introns belonging to ... between domain V and one of the intron–exon ...