Resuspend the protoplasts gently using electroporation buffer in an appropriate volume to obtain 2–5 × 10 6 protoplasts per ml based on counting with a hemacytometer.

Please use one of the following formats to cite this article in your essay, paper or report: APA. Shaffer, Catherine. (2023, July 19). Using Electroporation to Transform Bacteria, Yeast and Plant ...