Here, the authors describe genetically engineering the Pseudomonas genome by two-step allelic exchange. Suicide vector-encoded alleles are used to generate mutations by homologous recombination at ...

These constructs were co-transformed with the AcdA9 aminoacyl tRNA synthetase/tRNA-containing plasmid (AcdA9.pDule2) into B-95.ΔA E. coli (DE3) cells . 1 L cultures of transformed E. coli were grown ...

Figure 1. The vitamin K cycle and its link to carboxylation of glutamic acid residues on vitamin K–dependent coagulation proteins. Vitamin K 1 obtained from food sources is reduced to vitamin KH 2 by ...

Briefly, the plasmid POE-W I40 was transformed into BL21(DE3) E. coli and plated on 2XYT solid medium + carbenicillin. Starter cultures of nutrient-rich liquid medium + carbenicillin were inoculated ...

This study showed that all V. vulnificus isolates were susceptible to chloramphenicol, tetracycline, and nalidixic acid, and were all resistant to ampicillin, amoxicillin, carbenicillin, colistin, ...

Nicotiana benthamiana leaves were infiltrated with Agrobacterium tumefaciens GV3101 (grown in LB media supplemented with 50 μg/ml Carbenicillin and 20 μg/ml Gentamicin) carrying the respective ...

Cell envelope plays a dual role in the life of bacteria by simultaneously protecting it from a hostile environment and facilitating access to beneficial molecules. At the heart of this ability lie the ...