In this manuscript the authors established a novel three-dimensional culture system for stratified epithelia that allows epithelial cells to undergo epithelial-to-mesenchymal transition (EMT) and ...

The reactions were stopped by addition of equal volumes of 2X SDS-PAGE loading buffer (100 mM Tris pH 6.8, 10% v/v β-mercaptoethanol,4% v/v SDS, 0.2% v/v bromophenol blue, 20% v/v glycerol). Reactions ...

Loading buffer (40% glycerol, 240 mmol/L Tris-HCl pH 6.8, 8% SDS, 0.04% bromophenol blue, 50 mmol/L dithiothreitol) was added and the samples boiled at 95 °C for 5 minutes. The proteins were separated ...

Dye decolourisation was determined by measuring the decrease in absorbance using the following wavelengths for the different dyes: alizarin (496 nm), acid red 27 (520 nm), Congo red (490 nm), ...

Approximately 50 μg of each sample was diluted with 2 x Laemmli’s loading buffer (60 mM Tris–HCl pH 6.8, 10% glycerol, 2% SDS, 50 mM dithiothreitol, and 0.01% bromophenol blue). The protein samples ...

A ratio of damaged cells in a population was determined by counting images of smears stained with azure-eosin (Romanovsky-Giemsa method), bromophenol blue for evaluating cell protein content [13,14], ...

Blue Lock just kicked off a brand-new collaboration with the renowned English soccer team Liverpool FC! Blue Lock is currently one of the most hyped soccer anime series, and has been investing in ...

Blue Origin, the rocket company Amazon billionaire Jeff Bezos founded, notched one of the most substantial successes in its history on Thursday: sending a rocket to orbit. The debut flight of New ...

Jeff Bezos's space company Blue Origin has successfully launched its rocket into orbit on its first test flight. New Glenn - described by Blue Origin as a "giant, reusable rocket" and designed for ...

Here’s how it works. COCOA BEACH, Florida — Blue Origin's New Glenn rocket just earned its wings. New Glenn launched for the first time ever this morning (Jan. 16), rising off a pad at ...