Points at which a spike-in or exogenous control molecule can be added to a two-step RT-qPCR process are displayed. Inhibitors are most concentrated in extracted RNA and are gradually diluted out ...

Developing a reliable and sensitive qPCR assay, however, remains a challenging process. In this Q&A Stephen Bustin Professor of Molecular Biology at Anglia Ruskin University, Chelmsford, UK, and ...

Dr. Kavanagh shared his insights on what can go wrong in the qPCR process. Among the problem areas he cited was pipetting, nucleic acid isolation, RNA degradation, loss of sample, DNA ...

In addition, the complex RT-qPCR process might also reduce clinical sensitivity, resulting in false negative results. Recently, Guo and coworkers from Peking University developed an unprecedented ...