We cloned and sequenced the product, and found that both ligation junctions had a single copy of the expected junction sequence in all 11 clones sequenced. DNA-overhang cloning (DOC).

In diseases due to exposure to toxic particles like gout, macrophages elicit separate pathways for inflammation and lysosomal ...

Human exposure to toxic particles drives various diseases. Examples include gout, an acute arthritis driven by monosodium urate ...

Human exposure to toxic particles drives various diseases. Examples include gout, an acute arthritis driven by monosodium ...

At present, next-generation sequencing platforms use slightly different technologies for sequencing, such as pyrosequencing, sequencing by synthesis or sequencing by ligation. However, most ...

Imagine that you want to know the plot of a movie, but you only have access to either the visuals or the sound. With visuals ...

The tumor suppressor p53 is a transcription factor that controls the expression of hundreds of genes. Emerging evidence suggests that the p53-induced RNA-binding protein ZMAT3 is a key splicing ...

By combining artificial intelligence with automated robotics and synthetic biology, researchers have dramatically improved ...

While it is accepted that extracellular vesicles (EVs)-mediated transfer of microRNAs contributes to intercellular communication, the knowledge about molecular mechanisms controlling the selective and ...

The predominately used platforms use emulsion-PCR or bridge-PCR prior to pyrosequencing, sequencing by ligation or sequencing by synthesis. [8] ...

SNMMI annual meeting featured a prostate cancer session and a presentation by Dr. Sabina Dizdarevic discussing sequencing and combining beta and alpha targeted radionuclide treatments in the ...

Attention Leaving Cert Class of 2025 - you have come to the right place! RTÉ Learn is here to help with exam tips, free revision notes and video content. Click on the hyperlinks in this article ...