Somatic hypermutation (SHM) of immunoglobulin variable (V) regions modulates antibody-antigen affinity is initiated by activation-induced cytidine deaminase (AID) on single-stranded DNA (ssDNA).

The highly processive and novel warm-start reverse transcriptase allows scientists to run qPCR and PCR experiments without ice either right on the bench or out in the field. Once the lyophilized eQo ...

0.5 µL of reverse transcriptase, 1-5 µL of the RNA sample, and water to final volume of 10 µL. The RT reaction mixture was incubated at 25°C for 20 min followed by 37°C for 60 min, and finally heat ...

troubleshoot experiments, design primers and determine reaction conditions. We will cover critical requirements for amplification, thermostable DNA polymerases, reverse transcriptase reactions, ...

This could give rise to long-term expression of viral sequences, detectable by PCR ... elevated the level of reverse transcriptase.” The Whitehead team is pursuing experiments to confirm ...

There is interest in understanding the mechanisms that underlie reports that patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain PCR positive many ... conducted ...

In the two-step method, the reverse transcription and the qPCR amplification occur sequentially as two separate experiments. In the one-step method, RT and qPCR are performed in the same tube. Digital ...

For this reason, our initial strand-displacement experiments with D732N employed ... step of 30 min at 68°C was employed to allow the reverse transcription, followed immediately by programmed PCR ...

These two key events of the viral life cycle are commonly viewed as separate not only in time, but also in cellular space, since reverse transcription (RT ... Data are from one experiment. For CPSF6, ...

Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea SARS-CoV-2 real-time reverse-transcription PCR (rRT-PCR ... concentration ranges ...

Whereas a traditional PCR can take around 3 to 4 hours ... Combining a standard LAMP assay with reverse transcription (thus making it RT-LAMP) is possible by commercially available reverse ...