Introduction of artifacts is possible at any time during an array experiment, and so each component ... workers 35 used a modified real-time RT–PCR method to evaluate array data.

Reverse transcription - polymerase chain reaction (RT-PCR ... In order to determine the analytical sensitivity of the assay, RT-PCR experiments were performed on ten-fold dilution series of a viral ...

Somatic hypermutation (SHM) of immunoglobulin variable (V) regions modulates antibody-antigen affinity is initiated by activation-induced cytidine deaminase (AID) on single-stranded DNA (ssDNA).

Molecular methods, such as reverse transcriptase- polymerase chain reaction (RT-PCR), allow the development of rapid, sensitive and specific detection of AMPV (BÄYON-AUBOYER et al., 1999; D'ARCE et al ...