Efforts to estimate the reproducibility of published research results have focused on specific areas of research to date, ...

This manuscript characterizes a mutated clone of RNA polymerase I in yeast, referred to as SuperPol, to understand the mechanisms of RNA polymerase I elongation and termination. The authors present ...

In particular, real-time reverse transcription (RT)-PCR has become ... standardized protocols and controls to use in real-time PCR experiments. Another property of PCR that can complicate ...

These findings need to be validated by performing "wet lab" experiments. Activities in the project will involve culture of Kytococcus sedentarius, isolation and analysis of RNA and genomic DNA, PCR, ...

For all experiments, macrophages were incubated in the ... and SYBR Green Pro Taq HS Pre-Mix qPCR Kit (AG11701) for reverse transcription and quantitative fluorescence PCR. For the quantitative ...

All broilers received a conventional commercial feed and water ad libitum until the end of the experiment. This experiment was ... of total RNA was reversely transcribed into cDNA with a reverse ...

PCR-Machines are classified depending on the capacity of PCR used such as Nested PCR or Reverse Transcriptase PCR (RT-PCR ), or Multiplex RT PCR. PCR-machines are used for replication of DNA ...

Samples were tested for influenza with the use of a quantitative reverse-transcriptase–PCR (RT-PCR) assay (see the Supplementary Appendix). The influenza virus titer was determined in the index ...

Current molecular assays that are based on nested reverse transcription PCR and real-time PCR provide tools for follow-up of hepatitis A infection. The nucleic acid sequencing of selected regions ...

Multiplex PCR is a powerful technique that enables the detection of ... He will also present examples of 6-plex, 8-plex and 12-plex experiments for copy number variation (CNV) analysis and pathogen ...