The enzyme RT of HIV-1 is responsible for copying the viral single-stranded RNA genome to double-stranded DNA (dsDNA ... acid duplex may slide past the polymerase active site 17, and a rearranged ...

The reverse transcriptase enzyme that catalyzes this reaction has RNase activity that then degrades the RNA portion of the hybrid. Subsequently, a single-stranded DNA molecule is synthesized by the ...

In the latest shake-up for scientific dogma, research has demonstrated that eukaryotic cells can convert RNA sequences back into DNA, through a misunderstood, ‘error-prone’ polymerase. Historically, ...

RT-PCR (reverse-transcriptase polymerase chain reaction): PCR is a method that allows logarithmic amplification of short DNA sequences within a longer, double-stranded DNA molecule. Gene expression ...

Prior to its release into the nuclease, the covalently bound viral reverse transcriptase/DNA polymerase is removed. The nicked HBV DNA is repaired by the human host cell's DNA repair machinery.

The discovery of reverse transcriptase is a story ... a protein called RNA polymerase was involved in reverse-translating RNA to DNA. The discovery was transformative. The prevailing belief ...

“The idea of having a motor that you put in reverse sounds very appealing to us, but it's much more efficient to throw the motor out,” Wuite explained. Unlike cars, a cell has multiple DNA polymerase ...

The editorial by Bartlett and Starczynski, entitled, "Quantitative Reverse Transcriptase Polymerase Chain Reaction and the Oncotype DX Test for Assessment of Human Epidermal Growth Factor Receptor 2 ...

More information: Kaiyang Zheng et al, Diversity of reverse-transcriptase-containing viruses through global metagenomics, ...

After removing the nucleos(t)ide analog reverse-transcriptase inhibitor monophosphate, the mode of DNA polymerization becomes active again (bottom). The P site and N site of HIV-1 reverse ...