All lytic enzymes except lysing enzyme did not produce protoplasts in both strains A and R. Lysing enzyme at the concentration rate of 15mg/ml was found optimum for the isolation of protoplasts in ...

protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M ...

2019; Rich-Griffin et al., 2020; Xu et al., 2021). These advantages have led to focusing on the development of protoplast isolation and transient gene expression system aimed at improving the ...

Hence, there is the need for the development of new varieties with a low or null content of STLs, as it would simplify the inulin purification process ... expression of CRISPR/Cas9 components after ...