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In phage display technology, exogenous DNA is inserted into the phage genome, which generates a fusion protein with the phage coat protein, facilitates expression and promotes biological activity.
The T7 mouse monoclonal antibody was purchased from Novagen (San ... Supernatants were stored at −80°C. 2.7 Construction of T7 Phage Display cDNA Libraries Total RNA was isolated using the RNeasy Midi ...
and phage‐display technologies for screening protein–ligand and protein–protein interactions. With the explosion of genome and metagenome sequencing surveys around the world, we are coming to realize ...
Using a high throughput method, we developed a T7 phage display cDNA library derived from mRNA isolated from bronchoalveolar lavage (BAL) cells and leukocytes of sarcoidosis patients. This complex ...
Phage display often uses a type of phage known as filamentous, so called because they are long and thin, making the display of many proteins across its surface possible.
primarily phage display. With funding from the Science and Engineering Research Board (Department of Science and Technology, Government of India), I am currently working on producing recombinant mouse ...
While phage display has been around since Smith started studying it in 1984 as an extension of modern microbiology, which started in the 1940s, its application to drug discovery started an upswing ...
Use of phage display biopanning as a tool to design CAR-T cells against glioma stem cells. ... Membranes were incubated with 0.2 μg/mL mouse anti-human CD3ζ (Santa Cruz Biotechnology, SC-166275, ...