Hydrophobic interactions play a major role in binding non-native substrate proteins in the central cavity of the bacterial chaperonin GroEL. The sequence of local conformational changes by which GroEL ...

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After growth to OD 600 of ~0.5, 2 g/l of arabinose was added to induce GroEL/GroES expression. At OD 600 ~1, the temperature was lowered to 17°C and protein expression induced with 0.1 mM IPTG for ~18 ...

We demonstrate the mode of action of the degraders on a challenging essential target, GroEL. The studies in bacteria are complemented by in vitro binding and structural studies. Expression of degrader ...

The k cat /K m values of the green wild-type, R147W, and G185S SCAD enzymes coexpressed with GroEL/ES were 33, 30, and 10 μM-1 s-1, respectively. There were minimal differences in the kinetic ...