Furthermore, they removed a number of the CHO cells natural glycosylation genes using CRISPR-Cas9. This effort resulted in a cell line capable of producing over 1.2 grams pr. liter of cultured CHO ...

Unfortunately, CHO cells typically express lower levels of ... The data demonstrate highly comparable glycosylation patterns for antibody expressed in the ExpiCHO system or by stable CHO-S ...

generated in Chinese Hamster Ovary (CHO) cells and deficient in N-linked glycosylation sites vital for binding several families of anti-HIV broadly neutralizing antibodies (bN-mAbs). Researchers ...

However, the glycosylation modifications from these changes are rarely required for the function of a given product, and the additional glycans only occur at very low (<2%) levels that can be screened ...

such as CHO cells and mastocytoma cells, a tumorigenic variant of mast cells. To alter the glycosylation patterns of heparan sulfates, it is necessary to engineer their multi-enzyme biosynthetic ...

Significantly, the post-translational modifications (such as glycosylation) and folding of human proteins are more closely conserved when proteins are processed by CHO cells. A more recent ...

As target proteins, CHO cell lines expressing different types ... The relative amount of nine major glycosylation species was examined for six antibody products from the clarified samples.

Today, CHO-cells with the best yield produce around 10-12 g/L of monoclonal antibodies. Hence, this Alpha1-antitrypsin producing cell line is still only producing around 10 % of this known maximum.