Here, the authors describe genetically engineering the Pseudomonas genome by two-step allelic exchange. Suicide vector-encoded alleles are used to generate mutations by homologous recombination at ...

These constructs were co-transformed with the AcdA9 aminoacyl tRNA synthetase/tRNA-containing plasmid (AcdA9.pDule2) into B-95.ΔA E. coli (DE3) cells . 1 L cultures of transformed E. coli were grown ...

This study showed that all V. vulnificus isolates were susceptible to chloramphenicol, tetracycline, and nalidixic acid, and were all resistant to ampicillin, amoxicillin, carbenicillin, colistin, ...

Cell envelope plays a dual role in the life of bacteria by simultaneously protecting it from a hostile environment and facilitating access to beneficial molecules. At the heart of this ability lie the ...

The assembly of robust, modular biological components into complex functional systems is central to synthetic biology. Here, we apply modular “plug and play” design principles to a solid-phase protein ...