After that, 2 mL of MemGraft-Cy3-Biotin solution at 1 μM concentration in HBSS was ... prewashed Pierce Streptavidin Magnetic Beads from Thermo Fisher (because of toxic azide in the formulation), ...

Finally, all biotin-labeled proteins are eluted ... times with ice cold quenching buffer (PBS supplemented with 10 mM sodium ascorbate, 10 mM sodium azide, and 5 mM Trolox). The cells were detached by ...

On the second day, the sections were washed in PBS and incubated in biotinylated secondary antiserum (against appropriate species IgG, 1:1,000 in PBS) for 1 h, followed by a 1:1000 dilution of ...

Eggs are one of the richest sources of biotin, making them an excellent addition to your diet if you’re looking to promote hair growth. Eggs contain biotin in both the yolk and the white ...

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