To explore these questions, we determined the X-ray crystal structures of several representative β-lactams in their acyl−adduct complexes with the group I β-lactamase AmpC. A complex with the ...

The β-lactamases are grouped into classes A-D based on their amino acid sequence homology and enzyme mechanisms. Class A, C, and D β-lactamases are serine β-lactamases (SβLs) that inactivate β-lactam ...

The serine proteinase plasmin is the key fibrinolytic enzyme that dissolves blood clots and also promotes cell migration and tissue remodeling. Here, we report the 2.65 Å crystal structure of a ...

The molecules that form the foundation of life on Earth are as diverse as they are complex. Among these, carbohydrates play a ...

The solution to this challenge was provided by Alltech, courtesy of its ‘Digest P3’ enzyme complex. The company’s Adam Smyth was a recent visitor to the Lockharts’ AD operation.

The cysteine protease from the tobacco etch virus (TEVp) is a well-known and widely utilized enzyme. TEVp’s chymotrypsin-like fold is generally associated with serine catalytic triads that differ in ...

Metabolism of Exogenous D-Beta-Hydroxybutyrate, an Energy Substrate Avidly Consumed by the Heart and Kidney Bernard Cuenoud 1 * Mickaël Hartweg 2 Jean-Philippe Godin 3 Etienne Croteau 4 Mathieu ...

We propose that MAD2 NT is inserting the 5-LSR-7 segment midway into the narrow pore at the center of TRIP13 in the MAD2–p31-substrate–TRIP13 complex (Fig. 7E), while the 4-LTR-6 segment is captured ...

Similar hydrolysis curves have been reported for several protein substrates, such as peanut (Jamdar et al., 2010), Jatropha curcas seed cake (Selanon et al., 2014), rice protein (Li et al., 2016) and ...