The Wolves knocked off the Golden State Warriors 121-110 on Wednesday night to advance to the Western Conference finals ... narrowing New York’s series lead to 3-2. Boston won its first game ...

Total protein and RNA were extracted for western Blot analysis and quantitative polymerase chain reaction. Results and conclusions: bcl-2 specific siRNAs specifically inhibited expression of the ...

The Bcl-2 family controls apoptosis by direct interactions of ... For validation of knock-down efficiency cell lysates of transfected cells were analyzed by Western blot. Mutations were introduced in ...

SNU cells were harvested for detection of Bax, Bcl-2 and Caspase-3 antigen with flow cytometric analysis (FCM), immunocytochemical staining and Western blot. The expression of Bax and Caspase-3 in all ...

Furthermore, the influence of PEI-Pt NCs on apoptosis and its underlying mechanism was explored through the implementation of flow cytometry and Western blotting techniques ... apoptosis by modulating ...

In addition, the translational level of BCL ... BCL-2 antibody (Abcam, Cambridge, UK). Anti-mouse horseradish peroxidase was used as the secondary antibody (Cell Signaling Technology, Danvers, MA, USA ...

2.4. Western Blot Analysis Cells were harvested ... The target gene (CDK6, CyclinD1, and Bcl-2/Bax) expressions of A549 cells increased significantly in anti-miR-34a transfected cells, as shown in ...

Western blotting was performed as described in our previous study ... ab109301, Abcam, United States), and Bcl-2 (1:1,000, ER1706-47, Huabio, Hangzhou, China) at 4°C overnight, followed by 2-h ...