The perceived ease of use of real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) technology has revolutionized life science research. Its effectiveness at amplification and ...

Multiplex qPCR is a popular area of interest for many assay developers. Thanks to distinct reporter dyes, multiple reactions can be combined into a single well, with each target detected independently ...

View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to ...

We tested 2102 samples collected from 1237 persons up to 4 months after diagnosis by a quantitative polymerase-chain-reaction (qPCR) assay. We measured antibodies in 4222 quarantined persons who ...

He was investigating reports that using a technique called real-time quantitative PCR (qPCR) researchers had identified measles virus in intestinal tissue of children with developmental disorders 1.

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

A recently published paper in the Journal of Virological Methods is the first to directly compare ddPCR technology to RT-qPCR with multiplexed assays using the same reaction mix and cycling protocol ...

4. When reporting qPCR results, review the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, as described by Bustin, et al. 3 These guidelines ...

From detecting trace levels of virus to quantifying oncogenes in patient samples, quantitative PCR (qPCR) is the cornerstone of many diagnostic tests. At the basic research-level, scientists rely on ...