He was investigating reports that using a technique called real-time quantitative PCR (qPCR) researchers had identified measles virus in intestinal tissue of children with developmental disorders 1.

The perceived ease of use of real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) technology has revolutionized life science research. Its effectiveness at amplification and ...

View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to ...

A recently published paper in the Journal of Virological Methods is the first to directly compare ddPCR technology to RT-qPCR with multiplexed assays using the same reaction mix and cycling protocol ...

4. When reporting qPCR results, review the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, as described by Bustin, et al. 3 These guidelines ...

We tested 2102 samples collected from 1237 persons up to 4 months after diagnosis by a quantitative polymerase-chain-reaction (qPCR) assay. We measured antibodies in 4222 quarantined persons who ...

From detecting trace levels of virus to quantifying oncogenes in patient samples, quantitative PCR (qPCR) is the cornerstone of many diagnostic tests. At the basic research-level, scientists rely on ...