The team, at Universidad San Francisco de Quito in Ecuador, developed species- and genus-specific primers to detect the ...

“This is partly due to our implementation of a more sensitive testing method, qPCR, which allows us to test a larger number of mosquitoes, and hence detect any virus circulating in the populations.

“RT-qPCR is the gold standard for assessing presence ... test was the lack of understanding of the mutation rate of the virus and the location of mutation hot spots in the viral transcriptome ...

“Temperatures are rising around the world due to climate change and that is causing virus-transmitting mosquitos ... but syndromic qPCR testing identifies symptom-causing pathogens in one ...

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...