Whereas nonhomologous repair of DSBs is intact in these cells, homology-directed repair is significantly impaired. Homologous integration of transfected DNA is also reduced. Since these ES cells ...

Mol Cell Biol. 1998;18:93-101. Johnson RD, Liu N, Jasin M. 1999 Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination. Nature. 1999;401:397-399.

into the ROSA26 locus by homologous recombination in F1 (v6.5) ES cells. We used the ES-cell clone rtTA2ΔSD-18, which carried the targeted insert, as the donor for nuclear transplantation and ...