Efforts to estimate the reproducibility of published research results have focused on specific areas of research to date, ...

PCR-Machines are classified depending on the capacity of PCR used such as Nested PCR or Reverse Transcriptase PCR (RT-PCR ), or Multiplex RT PCR. PCR-machines are used for replication of DNA ...

In particular, real-time reverse transcription (RT)-PCR has become ... standardized protocols and controls to use in real-time PCR experiments. Another property of PCR that can complicate ...

To demonstrate the power of PCR–mediated recombination and provide ... as determined by both HIV–1 p24 antigen and reverse transcriptase assays. These particles were confirmed to be infectious ...

The development of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines to standardise qPCR methodology Challenges posed by reverse transcription ...

sensitivity and inhibitor resistance in reverse transcription loop-mediated isothermal amplification (RT-LAMP), RT-PCR, and other applications where the detection of low-quantities of target RNA from ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...