Scientists have now created a synthetic reverse transcriptase using directed evolution ... copying RNA into DNA and then amplifying the DNA with a DNA polymerase. But ...

PCR-Machines are based on Polymerase Chain ... such as Nested PCR or Reverse Transcriptase PCR (RT-PCR ), or Multiplex RT PCR. PCR-machines are used for replication of DNA, detecting DNA sequences ...

Once transcription is initiated, the DNA double helix unwinds and RNA polymerase reads the template strand, adding nucleotides to the 3′ end of the growing chain (Figure 2b). At a temperature of ...

It also contains telomerase reverse transcriptase (TERT), which uses TERC to synthesize and add telomeric DNA repeats to the ends of the chromosomes ... such as terminal restriction fragment (TRF), ...

The four DNA fragments (A, B ... as determined by both HIV–1 p24 antigen and reverse transcriptase assays. These particles were confirmed to be infectious virus by performing either co ...