RNA polymerases are sloppy. A retrovirus with an RNA genome, which is copied into DNA by a reverse transcriptase, is prone to mutations because the enzyme lacks the 3’ to 5’ proofreading capability of ...

The enzyme, called reverse transcriptase (RT), copies RNA molecules and changes them into complementary DNA duplicates that can then be inserted back into DNA, producing permanent sequence changes ...

The flap binds to a matching site, and the reverse transcriptase adds in the desired edit. The edited flap is incorporated back into the DNA, and with the help of a second nick on the complementary ...

2| Set up the following reverse transcription reaction and three controls: Incubate the reactions at 37 °C for 60 min. The success or failure of 5′ RACE is determined here at the beginning of ...