In addition, the Real-Time PCR Data Markup Language (RDML) has been developed by a consortium to enable the straightforward exchange of qPCR data and related information between qPCR instruments ...

RT-qPCR can be performed either as a coupled, one-step procedure (with RT and PCR performed in a single tube) or as a two-step procedure (with RT and PCR in separate tubes).

RT-qPCR testing, which detects SARS-CoV-2 genetic material present in a patient sample, quickly became the predominant method used to identify infected individuals. However, several claims and ...

RT-qPCR is a valuable tool but, as SARS-CoV-2 tests have shown, it has limits. How will researchers overcome them and what could it mean for diagnostics?

End-Stage Renal Disease (ESRD) is a severe chronic kidney disease with a rising global incidence, often accompanied by various complications, severely impacting patients' quality of life. Parthanatos ...

The initial stages of the RT-qPCR workflow, such as RNA isolation and reverse transcription, can critically impact the accuracy of the quantitation of gene expression. Learning Objectives: 1. Discuss ...

RNAConnect, developer of enzymatic tools for visualizing and manipulating RNA, today announced the release of the UltraMarathonRT® Two-Step RT-qPCR Kit, a next-generation tool for RNA researchers ...

CARLSBAD, Calif., July 1, 2025 /PRNewswire/ -- SeqOnce, a company focused on the research, development, and commercialization of qPCR and NGS genomics tools for the Infectious Disease Research &; CLIA ...

ICMR has approved a new RT-qPCR kit developed by Chennai-based KRIYA Medical technologies that can detect the presence or absence of Influenza, SARS CoV2, and Respiratory Syncytial Virus (RSV).

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription quantitative PCR, or RT-qPCR. In this process, RNA is isolated from samples of ...