Protoplast preparation, regeneration and fusion represent essential tools for those poorly studied biotechnologically valuable microorganisms inapplicable with the current molecular biology protocols.

Protoplast enzyme solution Dissolve 0.3 g cellulase, 0.3 g macerozyme, 0.12 g CaCl 2 ·2H 2 O in 30 ml wash buffer. Keep at 37 °C with occasional vortexing until the enzymes are completely ...

Research probing the secret of how some single plant cells can regenerate into whole plants will benefit from a new culture protocol developed by RIKEN scientists 1.. Some plants naturally possess the ...