No drug developer imagines that every kinase-targeting program can be advanced by any single kinase assay format. Most drug developers recognize that if they are to advance a kinase-targeting ...

Challenge of Traditional Assays An understanding of the diverse binding modes of kinase inhibitors and their effects on biological systems can provide researchers with important clues regarding ...

Interpretation of experiments that use these compounds is confounded by a lack of data on the comprehensive kinase selectivity of most inhibitors. Here we used functional assays to profile the ...

Multiple kinase activities can be measured by exposing a peptide array sequentially to different kinases. The assay also can be used to measure kinase inhibition. Results for the inhibition of the ...

Signal strength in this assay depends on length of time that the kinase reaction is allowed to proceed and the amount of enzyme used (Fig. 4). We have not observed saturation of the ...

Please click here for more information. Main use of outsourced kinase profiling assays: Figure 1: Therapeutic Areas Using Kinase Assays Today Around 2/3rd of all kinase profiling activities are ...

New kinase targets would lead to new drugs that could diversify ... The method utilizes a known imaging assay called bimolecular fluorescence complementation (BiFC) in which a fluorescent protein is ...

The Transcreener ADP 2 Assay uses a highly selective antibody and far-red fluorescent tracer to directly detect ADP produced by any ADP-producing enzyme (i.e. kinases, ATPases, helicases ...

Cisbio Bioassays has added three kinase assays to its growing cell-based kinase screening platform. The assays, all based on the company’s powerful HTRF® technology, were designed to provide new, easy ...

Purified proteins (100 ng per milliliter) were used in a LANCE Ultra kinase assay (PerkinElmer), with 100 nM ULight-poly GT (PerkinElmer) as a substrate and 200 μM ATP. Compounds were ...