Figure 4: Interphase defect in cnd2-1 at 36 °C monitored by Rad3-, Crb2- and Cds1-dependent checkpoint control. a , Mutant cnd2-1 incubated at 26 °C was transferred to 36 °C.

Spindle checkpoint activation by nocodazole treatment enhances the association between p55CDC and His6-BUBR1. ... Interphase (lane 7) and Noc-treated (lane 8) cell lysates were loaded as controls.