Did patients in a failed HIV vaccine trial halted in 2007 become more susceptible to the virus due to the adenoviral vector used to deliver the experimental vaccine? Researchers have speculated this ...

To determine the titre of MLV and HIV vector stocks, we used serial dilution of concentrated virus to infect 5×10 5 HeLa cells in a 6-well plate in the presence of polybrene (8 μgsol;ml).

Early steps of infection by HIV-1 involve entry of the viral core into cells, reverse transcription to form the linear viral DNA, and integration of that DNA into a chromosome of the host.